A BSC should be used for procedures involving open vessels of biohazards that:

A Biological Safety Cabinet (BSC) is essential for protecting both the user and the environment from exposure to biohazards. It creates a controlled work environment that helps contain aerosols and prevents the escape of harmful pathogens.

When considering procedures involving open vessels of biohazards, it's crucial to recognize that all the factors listed significantly increase the risk of exposure. Procedures that may produce infectious aerosols, especially when aerosol generation cannot be contained, pose a substantial danger. This is particularly relevant in a lab setting where ingestion or inhalation of infectious agents can occur. Additionally, working with high concentrations of biohazards raises the potential risk, as even a small volume can lead to significant contamination or exposure. Large volumes present their own set of challenges, as they connect to the potential for spills or splashes, which could compromise safety measures.

Using a BSC for all these scenarios ensures the implementation of an effective containment strategy. This is because the cabinet not only minimizes exposure but also filters out harmful particles from the air, thereby maintaining a sterile work environment. Therefore, it is important to use a BSC in all these situations to ensure comprehensive safety and compliance with best practices in handling biohazards.